Figure 4.Treatment with orexin-A ameliorated IL-1β-induced cellular senescence. Cells were stimulated with IL-1β (10 ng/ml) with or without orexin-A (5 and 10 μM) for 14 days, cellular senescence was measured using senescence-associated β-galactosidase (SA-β-gal) staining. (A) Representative images of SA-β-gal staining; (B) Quantification of SA-β-gal staining (##P < 0.01 vs. vehicle group; ^, ^^P < 0.05, 0.01 vs. IL-1β group, n = 5).
