Figure 1.HBx promoted proliferation, migration and invasion of hepatoma cells. (A) HBx overexpression promoted the expression of PCNA, CDH2, and snail by RT-qPCR detection. (B) HBx elevated the expression of proliferative migration markers by western blotting. (C, D) EdU and CCK-8 assays confirmed the effect of HBx overexpression on the proliferation of Huh-7 cells. (E, F) HBx overexpression also promoted migration and invasion by both wound-healing assays and Transwell. The experiments were repeated at least 3 independent times. (C–F) Data was presented as mean ± SD from three independent experiments. *p < 0.05; **p < 0.01.
