Figure 3.ANGPTL2 levels correlate with lung cancer cell migratory activity and facilitate LEC tube formation and migration. (A) The migratory ability of human lung cancer cell lines (CL1-0, CL1-5 and A549) was measured by the Transwell assay. (B, C) ANGPTL2 mRNA and protein expression in lung cancer cell lines was examined by Western blot (n=3) and qPCR. (D) Cells were transfected with ANGPTL2 cDNA or shRNA, then ANGPTL2 expression was measured by Western blot (n=3). *p < 0.05 compared with CL1-0 cells. (E–J) Cells were transfected with ANGPTL2 cDNA or shRNA. The CM was collected and applied to the LECs. LEC tube formation and migration was examined. *p < 0.05 compared with controls.
