Figure 6.ZCCHC12 promoted OS cells tumorigenicity in vivo. (A) Subcutaneous tumors were removed from nude mice and fixed in 0.4% formalin. (B and C), Tumor volume and weights of xenografts in nude mice were measured. (D), qRT-PCR was applied to examine the expression of ZCCHC12 in tumor xenograft tissues. (E), Western blot was performed to examine the expression of p-PI3K, p-AKT and ZCCHC12 in tumor xenograft tissues. (F) IHC staining for p-PI3K, p-AKT and ZCCHC12 in tumor xenograft tissues. scale bar: 200 μm. n = 5; ***P < 0.001.
