Figure 3 — Coupling of serum CK20 and hyper-methylated CLIP4 as promising biomarker for colorectal cancer diagnosis: from bioinformatics screening to clinical validation

Research Paper|Volume 13, Issue 24|pp. 26161–26179

Coupling of serum CK20 and hyper-methylated CLIP4 as promising biomarker for colorectal cancer diagnosis: from bioinformatics screening to clinical validation

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Figure 3.Specific overexpressed and de-methylated genes in CRC tissues were screened by bioinformatics and verified in CRC cell lines by Q-PCR or RT-PCR. (A) Screening specific overexpressed and de-methylated genes in CRC tissues by bioinformatics. (B) Testing the expression of de-methylated genes by Q-PCR in CRC cell lines and a normal colon cell line. The expression of de-methylated genes was tested using Q-PCR (C) and RT-PCR (D) in CRC cell lines after treatment with DAC.