Figure 2.The DNA methylation level at the second CpG site (Pos. 2) of the BMPR2 promoter was affected by the VHD clinicopathological factors. (A) The DNA methylation levels at Pos. 2 of BMPR2 were more significantly increased in the male patients than the females in comparison to the controls. (B) The DNA methylation levels at Pos. 2 of BMPR2 were shown in different age groups of VHD patients. (C) The DNA methylation levels at Pos. 2 of BMPR2 were more significantly increased in the VHD patients with more severe PAH than those with mild PAH. Moderate/severe PAH, PASP≥55mmHg. Mild PAH, 30 mmHg ≤ PASP < 55 mmHg. (D) The operation type of MVR or AVR or DVR had no effect on the BMPR2 promoter DNA methylation levels at Pos. 2 of BMPR2. MVR, mitral valve replacement; AVR, aortic valve replacement. DVR, double valve replacement. For statistical comparisons, *P < 0.05. **P < 0.01.
