Figure 6.Sev downregulated or increased the expression of key enzyme genes or proteins in glucose metabolism in the cortex or hippocampus. The mRNA expression of G6Pase and Pck1 (A, B); HK1 and PKM1 (E, F); Pdha1, Idh2, and Cs (I, J) in cortex and hippocampus was measured by real-time PCR. The expression of Pck1 (C, D), PKM1 (G, H) and Cs (K, L) protein in cortex and hippocampus was tested by western blot. Data are expressed as mean ±SD, n=5 (**p<0.01vs. control group, *p<0.05 vs. control group). (M, N) Showed the expression of Pck1 and Cs in cortex by immunofluorescence, respectively.
