Figure 1.Construction of cathartic colon mice models. (A) anatomical appearance of four groups. I saline group, II: morphine group, III rhubarb group, IV morphine+rhubarb group; Red A vermiform appendix, Red B rectum, Red C anus. (B) measurement of stool-transit distance after barium enema examination. I saline group (a) vs morphine group (b), II saline group (a) vs rhubarb group (b), III morphine group (a) vs morphine+rhubarb group (b), IV measurement of stool-transit distance; the forceps show the positions of transit barium, *p<0.05, versus saline group; #p<0.05, versus morphine group. (C) collection of stool samples. I appearance of stools in morphine group(L) and morphine+rhubarb group (R), II dynamic changes of the moisture content of stools in 65° C oven.
