Figure 1.macroH2A1 silencing (knock-down, KD) in UM 92.1 cells. (A) Cells were infected with a lentivirus bearing a bicistronic construct expressing GFP and a macroH2A1-silencing shRNA. Control cells (CTL) were infected with lentivirus bearing a bicistronic construct expressing GFP and a scramble shRNA (data not shown). (B) Western blot analysis showed a significant reduction of macroH2A1 in transfected cells.
