Figure 2.TGF-β1 induces fibrosis in HK2 cells. (A) qPCR analyses of E-cadherin (E-cad), ZO1, N-cadherin (N-cad) and α-SMA expression in HK2 cells treated with TGF-β1 at different concentrations for approximately 48 h. (B) Western blot analyses of E-cad, ZO1, N-cadherin and α-SMA expression in HK2 cells treated with TGF-β1 at different concentrations for approximately 48 h. GAPDH was used as a control. (C–E) CCK-8, EdU and cell migration analyses (transwell) of the viability, proliferation and migration of HK2 cells treated with TGF-β1 at different concentrations for approximately 48 h. *P < 0.05 and **P < 0.01.
