Figure 7.Effects of SIRT6 knockdown on viability of LUAD cells. (A) The SIRT6 protein expression was determined by western blot after SIRT6 knockdown. (B, C) NCI-H23 and A549 cell growth were determined by CCK8 assays and EdU staining assays. (D) Cell cycle was analyzed by flow cytometry. (E) The apoptotic rates of cells were detected by flow cytometry. (F, G) Cell ability of NCI-H23 and A549 cells co-transfection with si-SIRT6 and miR-138 inhibitor was detected by CCK8 and colony formation assays. (H, I) Cell ability of NCI-H23 and A549 cells co-transfection with si-SIRT6 and HMMR-AS1 was detected by CCK8 and colony formation assays. *P < 0.05, **P < 0.01.
