Figure 1 — Serines 440 and 467 in the Werner syndrome protein are phosphorylated by DNA-PK and affects its dynamics in response to DNA double strand breaks

Research Paper|Volume 6, Issue 1|pp. 70–81

Serines 440 and 467 in the Werner syndrome protein are phosphorylated by DNA-PK and affects its dynamics in response to DNA double strand breaks

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Figure 1.Mapping DNA-PK phosphorylation sites in WRN(A) Schematic representation of His- or GST-tagged WRN fragments used in in vitro phosphorylation assay. (B and C) In vitro phosphorylation assay. Purified His- or GST-tagged WRN fragments were incubated with purified DNA-PKcs, Ku 70/86, and activated DNA in the presence of [γ-32P]ATP. Amido black staining is shown (B). The phosphorylation was visualized (C). Asterisk indicates the GST (500-946) fragment. Note that GST (239-499) migrated slower because of many acidic amino acids.