Figure 4.Progerin suppresses the transcription activation of core adipogenic regulators during adipocyte differentiation(A) and (B) Quantitative RT-PCR analysis of early adipogenic genes C/EBPβ and C/EBPδ (A) and late adipogenic genes PPARγ2 and C/EBPα (B) in normal and HGPS iPSCs and adipocytes. n = 3. ** p < 0.01. (C) Western Blotting analysis with lamin A/C, PPARγ2, and actin antibodies in normal and HGPS iPSCs and adipocytes. Red filled triangle points to progerin. (D) Western Blotting analysis with C/EBPα and actin antibodies in normal and HGPS iPSCs and adipocytes. Cell lines: Normal (8470) and HGPS (164).
