Figure 7.BTP-2, a selective and potent inhibitor of SOCE reduces contractile force in young but not in aged skeletal muscle.(A) Intact EDL muscle stimulated with low and high frequencies every minute at 37°C; in control (no drug) contractions are shown, followed by the progressive effect of 20 μM BTP-2. On average the decrease in force with 20 μM it was 19 ± 4%. (B) Same protocol as in panel A, but using young SOL muscles, representative of 3 experiments. In these 3 experiments force decreased by 31 ± 7% in 15 min in SOL muscles. (C) EDL muscles from aged Wt mice were equilibrated with 30 and 80 Hz under control condition and then were exposed to 20 μM BTP-2. (D) SOL muscles from aged WT mice tested with the same protocol as shown for aged EDL muscles in panel C. No statistical differences between control and BTP-2 were seen in aged EDL and SOL muscles.
