Figure 3.Npm1/Sox2 interaction changes during differentiation.Induced differentiation of ES cells by withdrawal of leukemia inhibitory factor (LIF), addition of either dimethyl sulfoxide (DMSO) or retinoic acid (RA) for 24 h in combination with in situ PLA, showed that the Npm1/Sox2 complexes decreased during DMSO and leukemia inhibitory factor withdrawal induced differentiation. In contrast, retinoic acid induced differentiation did not notably affect the number of Npm1/Sox2 complexes in the nucleoplasm. DNA was counterstained with Hoechst 33342 (blue). Scale bar represents 10 μm.
