Figure 1. (A) No
difference in relaxation of aortic rings preconstricted with norepinephrine
to the vasodilator acetylcholine. % Relaxation = % of precontraction to
norepinephrine. (B) Relaxation of aortic rings at increasing sodium
nitroprusside concentrations after norepinephrine precontraction. %
Relaxation = % of precontraction to norepinephrine. (C) Contraction
of aortic rings at increasing norepinephrine concentrations. % Contraction
= % of contraction to 80 mM KCl. ApoE-/- SIRT1+/-
(blue line) and ApoE-/- SIRT1+/+ (black line).
(D) Aortic protein levels of total eNOS and phospho-eNOS (Ser1177). ApoE-/-
SIRT1+/- (+/- and black columns) and ApoE-/-
SIRT1+/+ (+/+ and white columns). n=6 per genotype
