Figure 4. Effect of DNA-PKcs on WRN helicase activity on
telomeric and non-telomeric D-loops. WRN wild type (WT) (3.3 nM,
lanes 5, 6, 12, and 13) or WRN (E84A) (3.3 nM, lanes 3, 4, 10, and 11)
was preincubated with DNA-PKcs (16.7 nM, lanes 4, 6, 11, and 13).
A telomeric (lanes 2-6) or a non-telomeric D-loop substrate (lanes 9-13)
was added to the reaction. Lanes 1 and 8: A DNA ladder marker. Lanes 7
and 14: heat-denatured telomeric and non-telomeric D-loop substrates,
respectively, denoted by filled triangles.
