Figure 2.Growth curves of young (3-4 mos), middle aged and old (20-24 mos) wild-type and Ames dwarf fibroblasts. (A)
Fibroblasts of young (3-4 mos) wild-type and Ames dwarf mice (3x104);
(B) middle aged (10-12 mos) wild-type and Ames dwarf mice (3x104)
and (C) old (20-24 mos) wild type and Ames dwarf mice (1x104)
were plated in triplicate in a 12-well culture plate. The cells from all
cultures were harvested and counted daily for 8 days. Each time-point
represents the average number of cells in 3 triplicated wells of cultures
established from 3 individual mice. N = 3; *p<0.05
between wild-type vs. dwarf mice.